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KMID : 1098420070150020105
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Korean Journal of Medicinal Crop Science
2007 Volume.15 No. 2 p.105 ~ p.111
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Neuroprotective Effect of Korean Mistletoe Extract against Damage Induced by Hydrogen Peroxide in Cultured Rat Cortical Neurons
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Lee Joo-Hyun
Cho Soon-Ock
Ban Ju-Yeon
Song Kyung-Sik
Seong Yeon-Hee
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Abstract
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The protective effect of ethanol extract of Korean mistletoe (KM; Viscum album coloratum) on hydrogen peroxide (H₂O₂)-induced neurotoxicity was examined in primary cultured rat cortical neurons. H₂O₂ reduced viability of cortical neurons in a concentration-dependent manner. The addition of KM, over a concentration range of 10 to 100 ㎍/ mL , concentration-dependently prevented the H₂O₂(100㎛)-induced neuronal cell death, as assessed by a 3-[4,5-dimethylthiazol-2-yl]-2,5-di-phenyl-tetrazolium bromide (MTT) assay and Hoechst 33342 staining. KM significantly inhibited (H₂O₂)-induced elevation of the cytosolic Ca²+ concentration (［ Ca²+］ｃ), which was measured by a fluorescent dye, fluo-4 AM. KM inhibited glutamate release into medium and generation of reactive oxygen species (ROS) induced by H₂O₂. These results suggest that KM may mitigate the H₂O₂-induced neurotoxiciy by interfering with the increase of ［ Ca²+］ｃ, and inhibiting glutamate release and generation of ROS in cultured neurons.
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KEYWORD
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Korean mistloetoe, neurotoxicity, hydrogen peroxide, neuroprotection, cortical neurons
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